Search results
Results from the WOW.Com Content Network
S. cerevisiae cells imaged by DIC microscopy A quantitative phase-contrast microscopy image of cells in culture. The height and color of an image point correspond to the optical thickness, which only depends on the object's thickness and the relative refractive index. The volume of an object can thus be determined when the difference in ...
The process of image production in a DIC microscope. The image has the appearance of a three-dimensional object under very oblique illumination, causing strong light and dark shadows on the corresponding faces. The direction of apparent illumination is defined by the orientation of the Wollaston prisms.
Phase-contrast imaging is the highest resolution imaging technique ever developed, and can allow for resolutions of less than one angstrom (less than 0.1 nanometres). It thus enables the direct viewing of columns of atoms in a crystalline material. [20] [21] The interpretation of phase-contrast images is not a straightforward task.
Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
The contrast of a high resolution transmission electron microscopy image arises from the interference in the image plane of the electron wave with itself. Due to our inability to record the phase of an electron wave, only the amplitude in the image plane is recorded.
This image shows a cross-section of the vascular tissue in a plant stem. Bright-field microscopy ( BF ) is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light , and contrast in the sample is caused by attenuation of the ...
The maximum contrast of an image is termed the contrast ratio or dynamic range. In images where the contrast ratio approaches the maximum possible for the medium, there is a conservation of contrast. In such cases, increasing contrast in certain parts of the image will necessarily result in a decrease in contrast elsewhere.
The optical transfer function (OTF) of an optical system such as a camera, microscope, human eye, or projector is a scale-dependent description of their imaging contrast. Its magnitude is the image contrast of the harmonic intensity pattern, + (), as a function of the spatial frequency, , while its complex argument indicates a phase shift ...