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Ion-exchange resin beads. An ion-exchange resin or ion-exchange polymer is a resin or polymer that acts as a medium for ion exchange, that is also known as an ionex. [1] It is an insoluble matrix (or support structure) normally in the form of small (0.25–1.43 mm radius) microbeads, usually white or yellowish, fabricated from an organic polymer substrate.
Anion-exchange chromatography is a process that separates substances based on their charges using an ion-exchange resin containing positively charged groups, such as diethyl-aminoethyl groups (DEAE). [2] In solution, the resin is coated with positively charged counter-ions . Anion exchange resins will bind to negatively charged molecules ...
Ion-exchange resin beads Ion-exchange column used for protein purification. Ion exchange is a reversible interchange of one species of ion present in an insoluble solid with another of like charge present in a solution surrounding the solid. Ion exchange is used in softening or demineralizing of water, purification of chemicals, and separation ...
Examples of functional groups of Weak ion exchange resins include diethylaminoethyl (DEAE, -C 2 H 4 N(C 2 H 5) 2), which is an anion exchanger, and carboxymethyl (CM, -CH 2-COOH), [35] which is a cation exchanger. These two types of exchangers can maintain the charge density of their columns over a pH range of 5–9.
DEAE-C is a weak anion exchanger. This exchange is utilized to separate proteins that have faintly differing charges. Like all anion exchangers, the resin carries a positive charge that interacts favorably with negative charges. The positive charge of DEAE cellulose is due to a protonated amine group.
The anion exchanger family (TC# 2.A.31, also named bicarbonate transporter family) is a member of the large APC superfamily of secondary carriers. [1] Members of the AE family are generally responsible for the transport of anions across cellular barriers, although their functions may vary. All of them exchange bicarbonate. Characterized protein ...
The resin has a higher affinity for highly charged countercations, for example by Ca 2+ (calcium) in the case of water softening. Correspondingly, anion-exchange resins are typically provided in the form of chloride Cl −, which is a highly mobile counteranion. Counterions are used in phase-transfer catalysis.
An ion-exchange membrane is generally made of organic or inorganic polymer with charged (ionic) side groups, such as ion-exchange resins. Anion-exchange membranes contain fixed cationic groups with predominantly mobile anions; because anions are the majority species, most of the conductivity is due to anion transport.