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A calibration curve plot showing limit of detection (LOD), limit of quantification (LOQ), dynamic range, and limit of linearity (LOL).. In analytical chemistry, a calibration curve, also known as a standard curve, is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration. [1]
In summary, in order to find a standard curve, one must use varying concentrations of BSA (Bovine Serum Albumin) [2] in order to create a standard curve with concentration plotted on the x-axis and absorbance plotted on the y-axis. Only a narrow concentration of BSA is used (2-10 ug/mL) in order to create an accurate standard curve. [23]
Each curve corresponds to a different Hill coefficient, labeled to the curve's right. The vertical axis displays the proportion of the total number of receptors that have been bound by a ligand. The horizontal axis is the concentration of the ligand. As the Hill coefficient is increased, the saturation curve becomes steeper.
A Bjerrum plot (named after Niels Bjerrum), sometimes also known as a Sillén diagram (after Lars Gunnar Sillén), or a Hägg diagram (after Gunnar Hägg) [1] is a graph of the concentrations of the different species of a polyprotic acid in a solution, as a function of pH, [2] when the solution is at equilibrium. Due to the many orders of ...
Equilibrium unfolding. In biochemistry, equilibrium unfolding is the process of unfolding a protein or RNA molecule by gradually changing its environment, such as by changing the temperature or pressure, pH, adding chemical denaturants, or applying force as with an atomic force microscope tip. [1][2] If the equilibrium was maintained at all ...
The Gompertz curve or Gompertz function is a type of mathematical model for a time series, named after Benjamin Gompertz (1779–1865). It is a sigmoid function which describes growth as being slowest at the start and end of a given time period. The right-side or future value asymptote of the function is approached much more gradually by the ...
The Standard addition method, often used in analytical chemistry, quantifies the analyte present in an unknown. This method is useful for analyzing complex samples where a matrix effect interferes with the analyte signal. In comparison to the calibration curve method, the standard addition method has the advantage of the matrices of the unknown ...
Standard solutions are commonly used to determine the concentration of an analyte species via calibration curve. A calibration curve is obtained by measuring a series of standard solutions with known concentrations, which can be used to determine the concentration of an unknown sample using linear regression analysis. [4]