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The foundation is governed by a board of trustees and awards grants for scientific research and scholarships. Alice C. Jantzen was the AOTF's first president, serving from 1965 to 1966. Publication
Lattice light-sheet microscopy is a modified version of light sheet fluorescence microscopy that increases image acquisition speed while decreasing damage to cells caused by phototoxicity. This is achieved by using a structured light sheet to excite fluorescence in successive planes of a specimen, generating a time series of 3D images which can ...
AOTF may refer to: Acousto-optic tunable filter, a piezoelectric optical device; American Occupational Therapy Foundation, a non-profit charitable, scientific and educational organization. Admiral of the Fleet, the highest rank in the British Royal Navy; AotF may refer to: The Age of the Fall (AF), a wrestling stable
Fluorescence recovery after photobleaching (FRAP) is a method for determining the kinetics of diffusion through tissue or cells. It is capable of quantifying the two-dimensional lateral diffusion of a molecularly thin film containing fluorescently labeled probes, or to examine single cells.
Recent biophotonics research has created new applications for clinical diagnostics and therapies involving fluids, cells, and tissues. These advances are allowing scientists and physicians opportunities for superior, non-invasive diagnostics for vascular and blood flow, as well as tools for better examination of skin lesions.
As well in the 1990s another super resolution microscopy method based on wide field microscopy has been developed. Substantially improved size resolution of cellular nanostructures stained with a fluorescent marker was achieved by development of SPDM localization microscopy and the structured laser illumination (spatially modulated illumination ...
IF can additionally be used in combination with other, non-antibody methods of fluorescent staining, e.g., the use of DAPI to label DNA. [10] [11] Examination of immunofluorescence specimens can be conducted utilizing various microscope configurations, including the epifluorescence microscope, confocal microscope, and widefield microscope. [12]
A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed. TIRFM is an imaging modality which uses the excitation of fluorescent cells in a thin optical specimen section that is supported on a glass slide.