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When the incident light beam is at Bragg angle, a diffraction pattern emerges where an order of diffracted beam occurs at each angle θ that satisfies: [3] = Here, m = ..., −2, −1, 0, +1, +2, ... is the order of diffraction, λ is the wavelength of light in vacuum, and Λ is the wavelength of the sound. [4]
The foundation is governed by a board of trustees and awards grants for scientific research and scholarships. Alice C. Jantzen was the AOTF's first president, serving from 1965 to 1966. Publication
AOTF may refer to: Acousto-optic tunable filter, a piezoelectric optical device; American Occupational Therapy Foundation, a non-profit charitable, scientific and educational organization. Admiral of the Fleet, the highest rank in the British Royal Navy; AotF may refer to: The Age of the Fall (AF), a wrestling stable
Lattice light-sheet microscopy is a modified version of light sheet fluorescence microscopy that increases image acquisition speed while decreasing damage to cells caused by phototoxicity. This is achieved by using a structured light sheet to excite fluorescence in successive planes of a specimen, generating a time series of 3D images which can ...
Microscope image processing is a broad term that covers the use of digital image processing techniques to process, analyze and present images obtained from a microscope. Such processing is now commonplace in a number of diverse fields such as medicine , biological research , cancer research , drug testing , metallurgy , etc.
Super-resolution optical fluctuation imaging (SOFI) is a post-processing method for the calculation of super-resolved images from recorded image time series that is based on the temporal correlations of independently fluctuating fluorescent emitters.
After clearing and labeling, tissues are typically imaged using confocal microscopy, [14] [15] [16] two-photon microscopy, [1] [5] [14] or one of the many variants of light-sheet fluorescence microscopy. [7] [14] [15] Other less commonly used methods include optical projection tomography [1] [5] and stimulated Raman scattering.
IF can additionally be used in combination with other, non-antibody methods of fluorescent staining, e.g., the use of DAPI to label DNA. [10] [11] Examination of immunofluorescence specimens can be conducted utilizing various microscope configurations, including the epifluorescence microscope, confocal microscope, and widefield microscope. [12]