Search results
Results from the WOW.Com Content Network
Mascot identifies proteins by interpreting mass spectrometry data. The prevailing experimental method for protein identification is a bottom-up approach, where a protein sample is typically digested with trypsin to form smaller peptides. While most proteins are too large, peptides usually fall within the limited mass range that a typical mass ...
Before analyzing with mass spectrometry, a protein must be accurately isolated and digested. If not isolated, the results will represent a mixture of two or more proteins and will therefore be unreliable in protein identification. Because of this sensitivity, sample preparation is likely the most important step in forming a peptide-mass ...
The MOWSE algorithm was developed by Darryl Pappin at the Imperial Cancer Research Fund and Alan Bleasby at the SERC Daresbury Laboratory. [2] The probability-based MOWSE score formed the basis of development of Mascot, a proprietary software for protein identification from mass spectrometry data.
A typical workflow of a peptide mass fingerprinting experiment. Peptide mass fingerprinting (PMF), also known as protein fingerprinting, is an analytical technique for protein identification in which the unknown protein of interest is first cleaved into smaller peptides, whose absolute masses can be accurately measured with a mass spectrometer such as MALDI-TOF or ESI-TOF. [1]
SEQUEST is a MS data analysis program used for protein identification. It correlates collections of tandem mass spectra to peptide sequences that have been generated from databases of protein sequences. [21] SIMS Open source: SIMS was designed to perform unrestricted PTM searches over tandem mass spectra. [22] SimTandem Freeware
Protein fingerprinting can refer to any of the several methods for identifying or differentiating proteins: Peptide mass fingerprinting, a method developed in 1993 that uses protein mass spectrometry following SDS-PAGE; Older techniques using two-dimensional chromatography and/or protein electrophoresis
These methods determined the mass of peptides using mass spectrometry, and then used the mass to search protein databases to identify the proteins [3] [4] In 1999 a more complex program was released called Mascot that integrated three types of protein/database searches: peptide molecular weights, tandem mass spectrometry from one or more ...
It contains de novo sequencing, database search, PTM identification, homology search and quantification in data analysis. Ma et al. described a new model and algorithm for de novo sequencing in PEAKS, and compared the performance with Lutefisk of several tryptic peptides of standard proteins, by the quadrupole time-of-flight (Q-TOF) mass ...