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Date/Time Thumbnail Dimensions User Comment; current: 12:22, 6 April 2014: 3 min 52 s, 480 × 360 (7.38 MB): Jacopo Werther == {{int:filedesc}} == {{Information |Description = Step-by-step video and audio instructions on how to prepare a wet mount specimen of eukaryotic animal cells; specifically Human epithelial cells from the inside of the cheek.
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A 40x magnification image of cells in a medical smear test taken through an optical microscope using a wet mount technique, placing the specimen on a glass slide and mixing with a salt solution. Optical microscopy is used extensively in microelectronics, nanophysics, biotechnology, pharmaceutic research, mineralogy and microbiology. [28]
Optical magnification is the ratio between the apparent size of an object (or its size in an image) and its true size, and thus it is a dimensionless number. Optical magnification is sometimes referred to as "power" (for example "10× power"), although this can lead to confusion with optical power.
A light micrograph or photomicrograph is a micrograph prepared using an optical microscope, a process referred to as photomicroscopy.At a basic level, photomicroscopy may be performed simply by connecting a camera to a microscope, thereby enabling the user to take photographs at reasonably high magnification.
In microscopy, the field of view in high power (usually a 400-fold magnification when referenced in scientific papers) is called a high-power field, and is used as a reference point for various classification schemes. For an objective with magnification , the FOV is related to the Field Number (FN) by
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A few tools have been developed to identify and analyze single cells during live-cell imaging. [ 2 ] [ 3 ] [ 4 ] Time-lapse microscopy is the method that extends live-cell imaging from a single observation in time to the observation of cellular dynamics over long periods of time.