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It describes the appearance of cells, tissues and cellular structures as seen through the microscope after a histological section has been stained with a basic dye. The most common such dye is haematoxylin. The name basophilic refers to the characteristic of these structures to be stained very well by basic dyes. This can be explained by their ...
In vivo staining (also called vital staining or intravital staining) is the process of dyeing living tissues. By causing certain cells or structures to take on contrasting colours, their form or position within a cell or tissue can be readily seen and studied.
Blood film stained with Giemsa showing Plasmodium (center of image), the parasite that causes malaria infections.. In 1891 Romanowsky [8] [9] [10] developed a stain using a mixture of eosin (typically eosin Y) and aged solutions of methylene blue that formed hues unattributable to the staining components alone: distinctive shades of purple in the chromatin of the cell nucleus and within ...
Staining dyes are dyes used for staining in microbiology and histology. Subcategories. This category has the following 2 subcategories, out of 2 total. ...
There are four basic steps of the Gram stain: Applying a primary stain ( crystal violet ) to a heat-fixed smear of a bacterial culture. Heat fixation kills some bacteria but is mostly used to affix the bacteria to the slide so that they do not rinse out during the staining procedure.
Trypan Blue, a living-cell exclusion dye; Triphenyl tetrazolium chloride; Erythrosine, which is Red No. 3 in food coloring, can be used as an exclusion dye. 7-Aminoactinomycin D used e.g. in flowcytometric studies of hematopoietic stem cell viability. Other Vital Stains : Janus Green B is a basic dye and vital stain used in histology.
It is used in biology and medicine as a histological stain. Cresyl violet is an effective and reliable stain used for light microscopy sections. Initially, tissue sections are "defatted" by passing through graded dilutions of ethanol. Then, rehydrated by passing back through decreasing concentrations of ethanol. Lastly, back into water.
Neutral red (toluylene red, Basic Red 5, or C.I. 50040) is a eurhodin dye used for staining in histology. It stains lysosomes red. [ 1 ] It is used as a general stain in histology, as a counterstain in combination with other dyes, and for many staining methods.