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It describes the appearance of cells, tissues and cellular structures as seen through the microscope after a histological section has been stained with a basic dye. The most common such dye is haematoxylin. The name basophilic refers to the characteristic of these structures to be stained very well by basic dyes. This can be explained by their ...
In vivo staining (also called vital staining or intravital staining) is the process of dyeing living tissues. By causing certain cells or structures to take on contrasting colours, their form or position within a cell or tissue can be readily seen and studied.
Staining dyes are dyes used for staining in microbiology and histology. Subcategories. This category has the following 2 subcategories, out of 2 total. ...
Calcofluor-white or CFW is a fluorescent blue dye used in biology and textiles. It binds to 1–3 beta and 1–4 beta polysaccharides of chitin and cellulose that are present in cell walls on fungi, plants, and algae. In plant cell biology research, it is used for the staining of cell walls of both algae and higher plants.
It is used in biology and medicine as a histological stain. Cresyl violet is an effective and reliable stain used for light microscopy sections. Initially, tissue sections are "defatted" by passing through graded dilutions of ethanol. Then, rehydrated by passing back through decreasing concentrations of ethanol. Lastly, back into water.
[2] [3] Carbol fuchsin is used as the primary stain dye to detect acid-fast bacteria because it is more soluble in the cells' wall lipids than in the acid alcohol. If the bacteria is acid-fast the bacteria will retain the initial red color of the dye because they are able to resist the destaining by acid alcohol (0.4–1% HCl in 70% EtOH). [4]
Trypan Blue, a living-cell exclusion dye; Triphenyl tetrazolium chloride; Erythrosine, which is Red No. 3 in food coloring, can be used as an exclusion dye. 7-Aminoactinomycin D used e.g. in flowcytometric studies of hematopoietic stem cell viability. Other Vital Stains : Janus Green B is a basic dye and vital stain used in histology.
A dye is a colored substance that chemically bonds to the substrate to which it is being applied. This distinguishes dyes from pigments which do not chemically bind to the material they color. Dye is generally applied in an aqueous solution and may require a mordant to improve the fastness of the dye on the fiber. [2]