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The hemocytometer (or haemocytometer, or Burker's chamber) is a counting-chamber device originally designed and usually used for counting blood cells. [ 1 ] The hemocytometer was invented by Louis-Charles Malassez and consists of a thick glass microscope slide with a rectangular indentation that creates a precision volume chamber.
Instrument Uses Flow cytometer: used for automated cell counting as in total blood count, differential count, etc. : Tissue bath or organ bath or Dale's apparatus: used in full tissue experiments, for example using guinea pig ileum mainly used in pharmacology for application of drugs to these tissues.
This type of hematology analyzer utilizes both Coulter's principle and flow cytometry to determine the granularity, diameter, and inner complexity of the cells. Using hydrodynamic focusing, the cells are sent through an aperture one cell at a time. During this, a laser is directed at them, and the scattered light is measured at multiple angles.
A counting chamber, is a microscope slide that is especially designed to enable cell counting. Hemocytometers and Sedgewick Rafter counting chambers are two types of counting chambers. The hemocytometer has two gridded chambers in its middle, which are covered with a special glass slide when counting.
Cytometers are the instruments which count the blood cells in the common blood test.. Cytometry is the measurement of number and characteristics of cells.Variables that can be measured by cytometric methods include cell size, cell count, cell morphology (shape and structure), cell cycle phase, DNA content, and the existence or absence of specific proteins on the cell surface or in the ...
Examination of cells in body fluids was historically performed using a hemocytometer, a chamber designed for counting cells microscopically. [11] This technique was limited by poor discrimination between cell types (cells could only be classified as mononuclear or polymorphonuclear) and the low number of cells present in unconcentrated body fluids.
A Coulter counter [3] is one of the other devices used for cell counting. Like CASY technology, this also uses electric current for cell counting. However, the difference between them is that there is an aperture called “sensing zone”, with a known volume of electrolyte in a coulter counter.
A dilution of the cells to be counted is prepared and mixed with Trypan blue, this is normally the stain of choice because it is taken up by dead cells and actively excluded from live cells. Once the cells have been stained, they are counted using a hemocytometer, then a calculation is carried out to the original concentration of live cells. [1]