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A 4Pi microscope is a laser scanning fluorescence microscope with an improved axial resolution. With it the typical range of the axial resolution of 500–700 nm can be improved to 100–150 nm, which corresponds to an almost spherical focal spot with 5–7 times less volume than that of standard confocal microscopy .
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A reference dimension is a dimension on an engineering drawing provided for information only. [1] Reference dimensions are provided for a variety of reasons and are often an accumulation of other dimensions that are defined elsewhere [2] (e.g. on the drawing or other related documentation). These dimensions may also be used for convenience to ...
Download as PDF; Printable version; In other projects Wikidata item; Appearance. move to sidebar hide. 4Pi may refer to: IBM System/4 Pi, a family of avionics ...
A standard ruler is an astronomical object for which the actual physical size is known. By measuring its angular size in the sky, one can use simple trigonometry to determine its distance from Earth. In simple terms, this is because objects of a fixed size appear smaller the further away they are.
The calculator described above was called "Model No. 1" . [6] Model 2 had scales on the inner cylinder for calculating logs and sines.The "Fuller-Bakewell" model 3 had two scales of angles printed on the inner cylinder to calculate cosine² and sine ⋅ cosine [note 1] for use by engineers and surveyors for tacheometry calculations.
An architect's scale is a specialized ruler designed to facilitate the drafting and measuring of architectural drawings, such as floor plans and Multi-view orthographic projections. Because the scale of such drawings is often smaller than life-size, an architect's scale features multiple units of length and proportional length increments. [1]
By 1978, the first theoretical ideas had been developed to break the Abbe limit, which called for using a 4Pi microscope as a confocal laser-scanning fluorescence microscope where the light is focused from all sides to a common focus that is used to scan the object by 'point-by-point' excitation combined with 'point-by-point' detection. [14]