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Sperm Chromatin Structure Assay (SCSA) is a diagnostic approach that detects sperm abnormality with a large extent of DNA fragmentation. [1] First described by Evenson in 1980, the assay is a flow cytometric test that detects the vulnerability of sperm DNA to acid-induced denaturation DNA in situ. [2] SCSA measures sperm DNA fragmentation ...
The most common reasons for laboratory semen analysis in humans are as part of a couple's infertility investigation and after a vasectomy to verify that the procedure was successful. [4] It is also commonly used for testing human donors for sperm donation, and for animals semen analysis is commonly used in stud farming and farm animal breeding.
Electroejaculation is a procedure used to obtain semen samples from sexually mature male mammals. The procedure is used for breeding programs and research purposes in various species, as well as in the treatment of ejaculatory dysfunction in human males. This procedure is used frequently with large mammals, particularly bulls and some domestic ...
Sperm washing is a standard procedure in infertility treatment. Once the fastest sperm have been isolated, before using them for artificial insemination or in vitro fertilization, it is important to confirm the absence of HIV virus in the sample. The sample obtained after washing is analysed, usually using the PCR technique, to check that there ...
Diff-Quik is a commercial Romanowsky stain variant used to rapidly stain and differentiate a variety of pathology specimens. It is most frequently used for blood films and cytopathological smears, including fine needle aspirates. [1][2][3] The Diff-Quik procedure is based on a modification of the Wright-Giemsa stain pioneered by Harleco in the ...
Hypospermia is a condition in which a man has an unusually low ejaculate (or semen) volume, less than 1.5 mL. It is the opposite of hyperspermia, which is a semen volume of more than 5.5 mL. [1] It should not be confused with oligospermia, which means low sperm count. Normal ejaculate when a man is not drained from prior sex and is suitably ...
Histopathology of various spermatogenesis disorders.. The diagnosis of oligozoospermia is based on one low count in a semen analysis performed on two occasions. For many decades sperm concentrations of less than 20 million sperm/ml were considered low or oligospermic, recently, however, the WHO reassessed sperm criteria and established a lower reference point, less than 15 million sperm/ml ...
Sperm sorting is a means of choosing what type of sperm cell is to fertilize the egg cell. Several conventional techniques of centrifugation or swim-up. Newly applied methods such as flow cytometry expand the possibilities of sperm sorting and new techniques of sperm sorting are being developed. It can be used to sort out sperm that are most ...
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