Search results
Results from the WOW.Com Content Network
Gene length: Longer genes will have more fragments/reads/counts than shorter genes if transcript expression is the same. This is adjusted by dividing the FPM by the length of a feature (which can be a gene, transcript, or exon), resulting in the metric fragments per kilobase of feature per million mapped reads (FPKM). [90]
Sequencing technologies vary in the length of reads produced. Reads of length 20-40 base pairs (bp) are referred to as ultra-short. [2] Typical sequencers produce read lengths in the range of 100-500 bp. [3] However, Pacific Biosciences platforms produce read lengths of approximately 1500 bp. [4] Read length is a factor which can affect the results of biological studies. [5]
The restriction fragments are then ligated together. [31] A molecular marker is then generated when specific fragments are selected for amplification. AFLP markers are run alongside a DNA marker on a gel. A common AFLP DNA marker is 30-330bp long. [32] The fragments of this marker lie at 10bp intervals to increase precision. RAPD
The size of restriction fragments determines the resolution of interaction mapping. Restriction enzymes (REs) that make cuts on 6bp recognition sequences, such as EcoR1 or HindIII, are used for this purpose, as they cut the genome once every 4000bp, giving ~ 1 million fragments in the human genome.
[4] [16] [17] Fragments of this size are suitable for high-throughput sequencing. [4] [16] [17] Following sonication, fragments can be size selected using AMPure XP beads from Beckman Coulter to obtain ligation products with a size distribution between 150 and 300 bp. [4] [17] This is the optimal fragment size window for HiSeq cluster formation ...
An example of the fragmentation of a protocol data unit in a given layer into smaller fragments. IP fragmentation is an Internet Protocol (IP) process that breaks packets into smaller pieces (fragments), so that the resulting pieces can pass through a link with a smaller maximum transmission unit (MTU) than the original packet size.
Maximum subarray problems arise in many fields, such as genomic sequence analysis and computer vision.. Genomic sequence analysis employs maximum subarray algorithms to identify important biological segments of protein sequences that have unusual properties, by assigning scores to points within the sequence that are positive when a motif to be recognized is present, and negative when it is not ...
The maximum segment size (MSS) is a parameter of the Options field of the TCP header that specifies the largest amount of data, specified in bytes, that a computer or communications device can receive in a single TCP segment. It does not count the TCP header or the IP header (unlike, for example, the MTU for IP datagrams).