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MCF-7 Cells. MCF-7 is a breast cancer cell line isolated in 1970 from a 69-year-old White woman. [1] MCF-7 is the acronym of Michigan Cancer Foundation-7, referring to the institute in Detroit where the cell line was established in 1973 by Herbert Soule and co-workers. [2]
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The cells have an epithelial morphology and can metastasize in xenograft models. [1] When transducted with viral vectors carrying the p53 gene, HCT116 cells remain arrested in the G1 phase. [3] The proliferation of HCT116 colonies was found to be inhibited by 5-Fu/P85 copolymer micelles. [4]
A karyotype is the general appearance of the complete set of chromosomes in the cells of a species or in an individual organism, mainly including their sizes, numbers, and shapes.
Many methods are used to identify cell lines, including isoenzyme analysis, human lymphocyte antigen (HLA) typing, chromosomal analysis, karyotyping, morphology and STR analysis. [35] One significant cell-line cross contaminant is the immortal HeLa cell line. HeLa contamination was first noted in the early 1960s in non-human culture in the USA.
Morphology of the embryo is studied in scientific research and often marked by stages. A flow chart summarizing the stages in morphology of the embryo. For further details of the processes that occur within each stage, see Human embryogenesis. 1. Fertilization. After the egg is fertilized by the sperm, a zygote (single diploid cell) is formed.
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Chart showing the lineages of hematopoiesis. In the adjacent image, CFU-GEMM is the scientific name for the "common myeloid progenitor" that is responsible for forming all the cells of the myeloid lineages. As observed in the image, CFU-GEMM is capable of producing a diverse set of cells.
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