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Gel permeation chromatography (GPC) [1] is a type of size-exclusion chromatography (SEC), that separates high molecular weight or colloidal analytes on the basis of size or diameter, typically in organic solvents. The technique is often used for the analysis of polymers.
Gel permeation chromatography is often used in combination with multi-angle light scattering (MALS), Low-angle laser light scattering (LALLS) and/or viscometry for an absolute determination (i.e., independent of the chromatographic separation details) of the molecular weight distribution as well as the branching ratio and degree of long chain ...
There were also attempts to fractionate synthetic high polymers; however, it was not until 1964, when J. C. Moore of the Dow Chemical Company published his work on the preparation of gel permeation chromatography (GPC) columns based on cross-linked polystyrene with controlled pore size, [15] that a rapid increase of research activity in this ...
Gel permeation chromatography (GPC)—separation of synthetic polymers (aqueous or organic soluble). GPC is a powerful technique for polymer characterization using primarily organic solvents. Gel filtration chromatography (GFC)—separation of water-soluble biopolymers. GFC uses primarily aqueous solvents (typically for aqueous soluble ...
Polymer Char was founded by B. Monrabal in 1992 in the Valencia Technology Park, in Spain, being registered with the name of Polymer Characterization, S.A. . Its initial goal was to develop a commercial Crystallization Analysis Fractionation (CRYSTAF) instrument based on technology developed by Monrabal at Dow Chemical Company laboratories in the Netherlands to measure chemical composition ...
Determination of the Low Molecular Weight Content of a Polymer Using Gel Permeation Chromatography 120: Solution/Extraction Behaviour of Polymers in Water 121: Estimation of the adsorption coefficient (K OC) on Soil and on sewage sludge using High Performance Liquid Chromatography (HPLC) 122: Determination of pH, Acidity and Alkalinity: 123
The rejection can be determined in various ways and provides an indirect measurement of the pore size. One possibility is the filtration of macromolecules (often dextran, polyethylene glycol or albumin), another is measurement of the cut-off by gel permeation chromatography. These methods are used mainly to measure membranes for ultrafiltration ...
Gel electrophoresis is an electrophoresis method for separation and analysis of ... have lower resolving power for DNA but a greater range of separation, and are ...
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