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Thin-layer chromatography (TLC) is a chromatography technique that separates components in non-volatile mixtures. [1] It is performed on a TLC plate made up of a non-reactive solid coated with a thin layer of adsorbent material. [2] This is called the stationary phase. [2]
High-performance thin-layer chromatography (HPTLC) serves as an extension of thin-layer chromatography (TLC), offering robustness, simplicity, speed, and efficiency in the quantitative analysis of compounds. [1] This TLC-based analytical technique enhances compound resolution for quantitative analysis.
Planar chromatography is a separation technique in which the stationary phase is present as or on a plane. The plane can be a paper, serving as such or impregnated by a substance as the stationary bed (paper chromatography) or a layer of solid particles spread on a support such as a glass plate (thin-layer chromatography).
Chiral chromatographic assay is the first step in any study pertaining to enantioselective synthesis or separation. This includes the use of techniques viz. gas chromatography (GC), high performance liquid chromatography (HPLC), chiral supercritical fluid chromatography (SFC), capillary electrophoresis (CE) [35] and thin-layer chromatography (TLC).
High-performance thin-layer chromatography is first used to separate the lipids by physical and chemical characteristics, then transferred to a blotting matrix before the oligosaccharides are detected by a specific binding protein (i.e. antibodies or lectins).
Gas chromatography-mass spectrometry (GC-MS) is a two-dimensional chromatography technique that combines the separation technique of gas chromatography with the identification technique of mass spectrometry. GC-MS is the single most important analytical tool for the analysis of volatile and semi-volatile organic compounds in complex mixtures. [7]
Analytical chemistry – Study of the separation, identification, and quantification of matter; Chemical process – A method or means of somehow changing one or more chemicals or chemical compounds. High-performance liquid chromatography – Technique in analytical chemistry; Unit operation – Basic step in a process
Chiral thin-layer chromatography is a variant of liquid chromatography that is employed for the separation of enantiomers. It is necessary to use either a chiral stationary phase or; a chiral additive in the mobile phase.