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Nessler's reagent, named after Julius Neßler (Nessler), is a 0.09 mol/L solution of potassium tetraiodomercurate(II) in 2.5 mol/L potassium hydroxide. This pale solution becomes deeper yellow in the presence of ammonia (NH 3). At higher concentrations, a brown precipitate derivative of Millon's base (HgO·Hg(NH 2)Cl) may form.
Nessler cylinders (also named color comparison cylinders or color comparing cylinders) are laboratory tubes with a fixed volume, made of glass with optically plane bottom. On the walls, there are marks of the nominal stroke volume (usually 100 ml) and possibly one halfway mark (usually 50 ml). Nessler cylinders with a capacity of 100 cc and 50 cc
Nessler tubes work on the same principle. There are also electronic automated colorimeters; before these machines are used, they must be calibrated with a cuvette containing the control solution. The concentration of a sample can be calculated from the intensity of light before and after it passes through the sample by using the Beer–Lambert ...
A Nessler cylinder is a simple type of colorimeter. [3] It is best known for use with Nessler's reagent but can be used for any colorimetric chemical test. In practice, a pair of tubes is used, set on a white background. One tube is filled with color reagent and a known quantity of sample to act as a reference.
In New York, he found that hundreds of copies of his machine were in use, but most did not work well and were unreliable. Nessler opened a shop on East 49th Street, and soon had salons in Chicago, Detroit, Palm Beach, Florida and Philadelphia. Nessler also developed a machine for home use that was sold for fifteen dollars.
The Jaffe reaction is a colorimetric method used in clinical chemistry to determine creatinine levels in blood and urine. In 1886, Max Jaffe (1841–1911) wrote about its basic principles in the paper Über den Niederschlag, welchen Pikrinsäure in normalem Harn erzeugt und über eine neue Reaction des Kreatinins in which he described the properties of creatinine and picric acid in an alkaline ...
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The equipment required is a colorimeter, some cuvettes and a suitable color reagent. The process may be automated, e.g. by the use of an AutoAnalyzer or by flow injection analysis . Recently, colorimetric analyses developed for colorimeters have been adapted for use with plate readers to speed up analysis and reduce the waste stream.