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Phenol red exists as a red crystal that is stable in air. Its solubility is 0.77 grams per liter (g/L) in water and 2.9 g/L in ethanol. [1] It is a weak acid with pK a = 8.00 at 20 °C (68 °F). A solution of phenol red is used as a pH indicator, often in cell culture.
For example, phenol red exhibits an orange color between pH 6.8 and pH 8.4. The transition range may shift slightly depending on the concentration of the indicator in the solution and on the temperature at which it is used.
The doubly deprotonated (In 2-) phenolate form (the anion form of phenol) gives the familiar pink color. In strongly basic solutions, phenolphthalein is converted to its In(OH) 3− form, and its pink color undergoes a rather slow fading reaction [6] and becomes completely colorless when pH is greater than 13.
Solution: The main components of a universal indicator, in the form of a solution, are thymol blue, methyl red, bromothymol blue, and phenolphthalein. This mixture is important because each component loses or gains protons depending upon the acidity or alkalinity of the solution being tested. It is beneficial to use this type of universal ...
Chlorophenol red is an indicator dye that changes color from yellow to violet in the pH range 5.4 to 6.8. [2] The pH of a substance is determined by taking the negative logarithm of the Hydronium ion concentration and the indictor changes color due to the dissociation of H + ions. [3] The lambda max is at 572 nm. [4]
Aqueous solutions of phenol are weakly acidic and turn blue litmus slightly to red. Phenol is neutralized by sodium hydroxide forming sodium phenate or phenolate, but being weaker than carbonic acid, it cannot be neutralized by sodium bicarbonate or sodium carbonate to liberate carbon dioxide. C 6 H 5 OH + NaOH → C 6 H 5 ONa + H 2 O
1% Phenol red solution 4 ml/L; pH: 7.6; Indications. When inoculated with a sample organism and allowed to incubate, the medium will change color from red to yellow ...
pH-dependent dye indicators such as Phenol Red induce a color change from pink to yellow when the pH value of the reaction decreases upon DNA amplification. [13] Due to its pronounced color change, this is the most commonly used readout for RT-LAMP assays. [13]
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