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The reported count is the number of colonies counted multiplied by the dilution used for the counted plate A high TVC count indicates a high concentration of micro-organisms which may indicate poor quality for drinking water or foodstuff. In food microbiology it is used as a benchmark for the evaluation of the shelf-life of foodstuffs [1]
Bacteriological water analysis is a method of analysing water to estimate the numbers of bacteria present and, if needed, to find out what sort of bacteria they are. It represents one aspect of water quality. It is a microbiological analytical procedure which uses samples of water and from these samples determines the concentration of bacteria ...
For a long time, flow cytometers utilized UV-excited dyes to examine bacterioplankton which could be used in either low-cost flow cytometers with limited sensitivity, or expensive flow cytometers with the high sensitivity needed to distinguish heterotrophic bacteria from autotrophs. The introduction of blue-excited dyes such as SYBR Green I ...
Colony-forming units are used to quantify results in many microbiological plating and counting methods, including: The pour plate method wherein the sample is suspended in a Petri dish using molten agar cooled to approximately 40–45 °C (just above the point of solidification to minimize heat-induced cell death).
A bacterial reference chart is used to determine the number of bacteria in the sample. Appropriate treatment is applied to the water source once abnormal levels of bacterial activity are noticed. Once water treatment is effective the bacterial count produced by the dip slide test should be low, approximately <10 4. [2]
The total number of living aerobic bacteria can be determined using a plate count agar which is a substrate for bacteria to grow on. The medium contains casein which provides nitrogen , carbon , amino acids , vitamins and minerals to aid in the growth of the organism.
Bacteria travel upstream quite easily. They will reach the reservoir of sterile medium quickly unless the liquid path is interrupted by an air break in which the medium falls in drops through air. Continuous efforts to remedy each defect lead to variations on the basic chemostat quite regularly. Examples in the literature are numerous.
for simultaneous solid and liquid cultures. McIntosh and Filde's anaerobic jar: production of anaerobic conditions for organisms that die in the presence of even little oxygen (anaerobiosis), e.g. tetanus bacteria Microscope: to observe microscopic specimens that cannot be seen by the naked eye. Microtitre plates: mostly used for ELISA: Microtome