Search results
Results from the WOW.Com Content Network
Origin of replication: Necessary for the replication and maintenance of the vector in the host cell. Promoter : Promoters are used to drive the transcription of the vector's transgene as well as the other genes in the vector such as the antibiotic resistance gene.
An expression vector has features that any vector may have, such as an origin of replication, a selectable marker, and a suitable site for the insertion of a gene like the multiple cloning site. The cloned gene may be transferred from a specialized cloning vector to an expression vector, although it is possible to clone directly into an ...
This gene can be cloned from a naturally occurring gene, [2] or synthetically constructed. [3] The vector can be delivered using physical, chemical or viral methods. [4] Typically, the vectors used in DNA constructs contain an origin of replication, a multiple cloning site, and a selectable marker. [2]
The origin of replication (also called the replication origin) is a particular sequence in a genome at which replication is initiated. [1] Propagation of the genetic material between generations requires timely and accurate duplication of DNA by semiconservative replication prior to cell division to ensure each daughter cell receives the full ...
The T-DNA portion of the binary vector is flanked by left and right border sequences and may include a transgene as well as a plant selectable marker. Outside of the T-DNA, the binary vector also contains a bacterial selectable marker and an origin of replication (ori) for bacteria. [4] Representative series of binary vectors are listed below.
A replicon is a region of an organism's genome that is independently replicated from a single origin of replication [citation needed].A bacterial chromosome contains a single origin, and therefore the whole bacterial chromosome is a replicon.
pBR322 is 4361 base pairs in length [1] and has two antibiotic resistance genes – the gene bla encoding the ampicillin resistance (Amp R) protein, and the gene tetA encoding the tetracycline resistance (Tet R) protein. It contains the origin of replication of pMB1, and the rop gene, which encodes a restrictor of plasmid copy number.
Vector (C++), a type in the C++ Standard Template Library; Euclidean vector, a geometric object with a direction and magnitude Vector graphics, images defined by geometric primitives as opposed to bitmaps; Vector monitor, a display device used for early computers; Vector game, any video game that uses a vector graphics display