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It was developed in 1954 by Japanese medical laboratory scientist Dr. Katsuya Kato (1912–1991). [6] [7] The technique was modified for use in field studies in 1972 by a Brazilian team of researchers led by Brazilian parasitologist Naftale Katz (b.1940), [8] [9] and this modification was adopted by the WHO as a gold standard for multiple helminth infections.
Viruses such as rotavirus can also be found in stools. [11] Other stool tests involve the detection of antibiotic resistance as to guide appropriate therapy, e.g. Clarithromycin resistance of Helicobacter pylori represents a major challenge in eradication therapy but the responsible bacterial genomic markers can be detected in stool using PCR ...
Entamoeba coli is a non-pathogenic species of Entamoeba that frequently exists as a commensal parasite in the human gastrointestinal tract. E. coli (not to be confused with the bacterium Escherichia coli) is important in medicine because it can be confused during microscopic examination of stained stool specimens with the pathogenic Entamoeba histolytica. [1]
[citation needed] The Kato technique (also called the Kato-Katz technique) is a laboratory method for preparing human stool samples prior to searching for parasite eggs. Eggs per gram is a laboratory test that determines the number of eggs per gram of feces in patients suspected of having a parasitological infection, such as schistosomiasis.
A duodenal biopsy may incidentally detect Giardia organisms, as in this H&E stained sample. [33] According to the CDC, the detection of antigens on the surface of organisms in stool specimens is the current test of choice for diagnosis of giardiasis and provides increased sensitivity over more common microscopy techniques. [34]
However, due to intermittent egg shedding, multiple stool samples may be necessary for an accurate diagnosis. The WHO recommends the Kato-Katz technique as the method of choice for fascioliasis diagnosis attending to its ease of use and reproducibility, and its enhanced sensitivity compared with the observation of eggs in fresh faeces.
Major groups of parasites include protozoans (organisms having only one cell) and parasitic worms (helminths). Of these, protozoans, including cryptosporidium, microsporidia, and isospora, are most common in HIV-infected persons. Each of these parasites can infect the digestive tract, and sometimes two or more can cause infection at the same time.
Merthiolate-Iodine-Formaldehyde (MIF) is a solution used in biomedical laboratories for concentration of stool samples prior to microscopic investigation for parasites. Concentration of stool is necessary in order to raise sensitivity of microscopy, as in non-concentrated samples the likelihood of finding equivalents of actually present ...
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