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Logarithmic dilution. A serial dilution is the step-wise dilution of a substance in solution, either by using a constant dilution factor, or by using a variable factor between dilutions. If the dilution factor at each step is constant, this results in a geometric progression of the concentration in a logarithmic fashion.
Fluorescein aqueous solutions, diluted from 10,000 to 1 parts-per-million in intervals of 10 fold dilution. At 1 ppm the solution is a very pale yellow. At 1 ppm the solution is a very pale yellow. As the concentration increases the colour becomes a more vibrant yellow, then orange, with the final 10,000 ppm a deep red colour.
The following formulas can be used to calculate the volumes of solute (V solute) and solvent (V solvent) to be used: [1] = = where V total is the desired total volume, and F is the desired dilution factor number (the number in the position of F if expressed as "1/F dilution factor" or "xF dilution"). However, some solutions and mixtures take up ...
Titer (American English) or titre (British English) is a way of expressing concentration. [1] Titer testing employs serial dilution to obtain approximate quantitative information from an analytical procedure that inherently only evaluates as positive or negative.
Typically, ten-fold dilutions are used, and the dilution series is plated in replicates of 2 or 3 over the chosen range of dilutions. Often 100 μL are plated but also larger amounts up to 1 mL are used. Higher plating volumes increase drying times but often do not result in higher accuracy, since additional dilution steps may be needed. [5]
A 10-fold dilution series of this suspension was made ranging from 10 7 to 10 0 CFUv/ml in 200 μl total volume of PMH, occupying eight wells of the 96-well plate. At this time, cells exposed to no antimicrobial agent were added to several wells of the plate from the culture kept on ice; these are termed the "input" controls, since they ...
In general, a 10-fold increase in tris buffer concentration will lead to a 0.05 unit increase in pH and vice versa. [9] Silver-containing single-junction pH electrodes (e.g., silver chloride electrodes) are incompatible with tris since an Ag-tris precipitate forms which clogs the junction. Double-junction electrodes are resistant to this ...
Semi-log plot of the Internet host count over time shown on a logarithmic scale. A logarithmic scale (or log scale) is a method used to display numerical data that spans a broad range of values, especially when there are significant differences between the magnitudes of the numbers involved.