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A metaphase cell positive for the bcr/abl rearrangement (associated with chronic myelogenous leukemia) using FISH. The chromosomes can be seen in blue. The chromosome that is labeled with green and red spots (upper left) is the one where the rearrangement is present. Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique ...
Being a new type of fluorescent nanoparticles, applications of CQD lie in the field of bioimaging and biosensing due to their biological and environmental friendly composition and excellent biocompatibility. [1] In order to survive the competition with conventional semiconductor quantum dots, a high quantum yield should be achieved.
DNA microarrays can be used to detect DNA (as in comparative genomic hybridization), or detect RNA (most commonly as cDNA after reverse transcription) that may or may not be translated into proteins. The process of measuring gene expression via cDNA is called expression analysis or expression profiling.
Quantum yield is defined by the fraction of excited state fluorophores that decay through fluorescence: where. Φf is the fluorescence quantum yield, kf is the rate constant for radiative relaxation (fluorescence), knr is the rate constant for all non-radiative relaxation processes. Non-radiative processes are excited state decay mechanisms ...
Checked. In chemistry, orbital hybridisation (or hybridization) is the concept of mixing atomic orbitals to form new hybrid orbitals (with different energies, shapes, etc., than the component atomic orbitals) suitable for the pairing of electrons to form chemical bonds in valence bond theory. For example, in a carbon atom which forms four ...
In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acid strand (i.e., a probe) to localize a specific DNA or RNA sequence in a portion or section of tissue (in situ) or if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH ...
To improve fluorescence quantum yield, quantum dots can be made with shells of a larger bandgap semiconductor material around them. The improvement is suggested to be due to the reduced access of electron and hole to non-radiative surface recombination pathways in some cases, but also due to reduced Auger recombination in others.
Resonance fluorescence is used primarily in the coherent control of atoms. By coupling a two-level atom, such as a quantum dot, to an electric field in the form of a laser, you are able to effectively create a qubit. The qubit states correspond to the excited and the ground state of the two-level atoms.