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By virtue of the linearity property of optical non-coherent imaging systems, i.e., . Image(Object 1 + Object 2) = Image(Object 1) + Image(Object 2). the image of an object in a microscope or telescope as a non-coherent imaging system can be computed by expressing the object-plane field as a weighted sum of 2D impulse functions, and then expressing the image plane field as a weighted sum of the ...
The three-dimensional point spread functions (a,c) and corresponding modulation transfer functions (b,d) of a wide-field microscope (a,b) and confocal microscope (c,d). In both cases the numerical aperture of the objective is 1.49 and the refractive index of the medium 1.52.
The Gaussian function has a 1/e 2 diameter (2w as used in the text) about 1.7 times the FWHM.. At a position z along the beam (measured from the focus), the spot size parameter w is given by a hyperbolic relation: [1] = + (), where [1] = is called the Rayleigh range as further discussed below, and is the refractive index of the medium.
The effect of the contrast transfer function can be seen in the alternating light and dark rings (Thon rings), which show the relation between contrast and spatial frequency. The contrast transfer function (CTF) mathematically describes how aberrations in a transmission electron microscope (TEM) modify the image of a sample.
Half width at half maximum (HWHM) is half of the FWHM if the function is symmetric. The term full duration at half maximum (FDHM) is preferred when the independent variable is time . FWHM is applied to such phenomena as the duration of pulse waveforms and the spectral width of sources used for optical communications and the resolution of ...
The pupil function or aperture function describes how a light wave is affected upon transmission through an optical imaging system such as a camera, microscope, or the human eye. More specifically, it is a complex function of the position in the pupil [ 1 ] or aperture (often an iris ) that indicates the relative change in amplitude and phase ...
To calculate back to φ e (x,u) the wave in the image plane is back propagated numerically to the sample. If all properties of the microscope are well known, it is possible to recover the real exit wave with very high accuracy. First however, both phase and amplitude of the electron wave in the image plane must be measured.
The standard luminous efficiency function is normalized to a peak value of unity at 555 nm (see luminous coefficient). The value of the constant in front of the integral is usually rounded off to 683 lm/W. The small excess fractional value comes from the slight mismatch between the definition of the lumen and the peak of the luminosity function.