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Although Maxam and Gilbert published their chemical sequencing method two years after Frederick Sanger and Alan Coulson published their work on plus-minus sequencing, [2] [3] Maxam–Gilbert sequencing rapidly became more popular, since purified DNA could be used directly, while the initial Sanger method required that each read start be cloned for production of single-stranded DNA.
Microfluidic Sanger sequencing is a lab-on-a-chip application for DNA sequencing, in which the Sanger sequencing steps (thermal cycling, sample purification, and capillary electrophoresis) are integrated on a wafer-scale chip using nanoliter-scale sample volumes. This technology generates long and accurate sequence reads, while obviating many ...
3)Sequencing: The amplified cDNA is then sequenced using a technique such as Sanger sequencing or Maxam-Gilbert sequencing. Challenges and Limitations. Traditional RNA sequencing methods have several limitations. For example: They require the creation of a cDNA molecule, which can be time-consuming and labor-intensive.
Sanger's approach was described in 2001 as one of the two fundamental methods for sequencing DNA fragments [1] (the other being the Maxam–Gilbert method [5]) but the Sanger method is both the "most widely used and the method used by most automated DNA sequencers."
The first DNA sequencing methods were developed by Gilbert (1973) [8] and Sanger (1975). [9] Gilbert introduced a sequencing method based on chemical modification of DNA followed by cleavage at specific bases whereas Sanger's technique is based on dideoxynucleotide chain termination. The Sanger method became popular due to its increased ...
Allan Maxam. Allan Maxam (born October 28, 1942) is one of the pioneers of molecular genetics. He was one of the contributors to develop a DNA sequencing method at Harvard University, while working as a student in the laboratory of Walter Gilbert. [1][2] Walter Gilbert and Allan Maxam developed a DNA sequencing method - now called Maxam-Gilbert ...
The DNA sequencing methods used in the 1970s and 1980s were manual; for example, Maxam–Gilbert sequencing and Sanger sequencing. Several whole bacteriophage and animal viral genomes were sequenced by these techniques, but the shift to more rapid, automated sequencing methods in the 1990s facilitated the sequencing of the larger bacterial and ...
The high demand for low-cost sequencing has driven the development of high-throughput sequencing technologies that parallelize the sequencing process, producing thousands or millions of sequences at once. [58] [59] High-throughput sequencing is intended to lower the cost of DNA sequencing beyond what is possible with standard dye-terminator ...