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This cell line was originally established from the primary mouse embryonic fibroblast cells that were cultured by the designated protocol, so-called '3T3 protocol'. The primary mouse embryonic fibroblast cells were transferred (the "T") every 3 days (the first "3"), and inoculated at the rigid density of 3 × 10 5 cells per 20 cm 2 dish (the ...
Mouse Embryonic Fibroblasts (MEFs) are a type of fibroblast prepared from mouse embryo. MEFs show a spindle shape when cultured in vitro, a typical feature of fibroblasts. The MEF is a limited cell line. After several transmissions, MEFs will senesce and finally die off.
Gail Roberta Martin (née Zuckman, born 1944) is an American biologist. She is professor emerita in the Department of Anatomy, University of California, San Francisco.She is known for her pioneering work on the isolation of pluripotent stem cells from normal embryos, for which she coined the term 'embryonic stem cells'. [1]
WI-38 is a diploid human cell line composed of fibroblasts derived from lung tissue of a 3-month-gestation female fetus. [ 1 ] [ 2 ] The fetus came from the elective abortion of a Swedish woman in 1963.
Differentiated adipocytes in a 3T3-L1 cell line stained with Oil Red O. 3T3-L1 is a sub clonal cell line derived from the original 3T3 Swiss albino cell line of 1962. The 3T3 original cell line was isolated from a mouse embryo and propagated for this specific line of 3T3 cells is used to study adipose tissue-related diseases and dysfunctions.
This early use of electrospun fibrous lattices for cell culture and tissue engineering showed that various cell types including Human Foreskin Fibroblasts (HFF), transformed Human Carcinoma (HEp-2), and Mink Lung Epithelium (MLE) would adhere to and proliferate upon polycarbonate fibers.
Fibroblasts can also migrate slowly over substratum as individual cells, again in contrast to epithelial cells. While epithelial cells form the lining of body structures, fibroblasts and related connective tissues sculpt the "bulk" of an organism. The life span of a fibroblast, as measured in chick embryos, is 57 ± 3 days. [4]
Transcription factor 21 (TCF21), also known as pod-1, capsuling, or epicardin, is a protein that in humans is encoded by the TCF21 gene on chromosome 6. [5] [6] [7] It is ubiquitously expressed in many tissues and cell types and highly significantly expressed in lung and placenta. [8]
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