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  2. Cell culture - Wikipedia

    en.wikipedia.org/wiki/Cell_culture

    Cell culture or tissue culture is the process by which cells are grown under controlled ... Cells can be cultured for a longer time if they are split regularly, as it ...

  3. Hayflick limit - Wikipedia

    en.wikipedia.org/wiki/Hayflick_limit

    The typical normal human fetal cell will divide between 50 and 70 times before experiencing senescence. As the cell divides, the telomeres on the ends of chromosomes shorten. The Hayflick limit is the limit on cell replication imposed by the shortening of telomeres with each division. This end stage is known as cellular senescence.

  4. Cell division - Wikipedia

    en.wikipedia.org/wiki/Cell_division

    Cell division over 42. The cells were directly imaged in the cell culture vessel, using non-invasive quantitative phase contrast time-lapse microscopy. [38] In 2022, scientists discovered a new type of cell division called asynthetic fission found in the squamous epithelial cells in the epidermis of juvenile zebrafish.

  5. Cell synchronization - Wikipedia

    en.wikipedia.org/wiki/Cell_synchronization

    Cell synchronization is a process by which cells in a culture at different stages of the cell cycle are brought to the same phase. Cell synchrony is a vital process in the study of cells progressing through the cell cycle as it allows population-wide data to be collected rather than relying solely on single-cell experiments.

  6. Fission (biology) - Wikipedia

    en.wikipedia.org/wiki/Fission_(biology)

    The new cell wall fully develops, resulting in the complete split of the bacterium. The new daughter cells have tightly coiled DNA rods, ribosomes, and plasmids; these are now brand-new organisms. Studies of bacteria made to not produce a cell wall, called L-form bacteria, shows that FtsZ requires a cell wall to work.

  7. Trypsinization - Wikipedia

    en.wikipedia.org/wiki/Trypsinization

    Trypsinization is the process of cell dissociation using trypsin, a proteolytic enzyme which breaks down proteins, to dissociate adherent cells from the vessel in which they are being cultured. When added to cell culture, trypsin breaks down the proteins that enable the cells to adhere to the vessel.

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  9. Confluency - Wikipedia

    en.wikipedia.org/wiki/Confluency

    In cell culture biology, confluence refers to the percentage of the surface of a culture dish that is covered by adherent cells.For example, 50 percent confluence means roughly half of the surface is covered, while 100 percent confluence means the surface is completely covered by the cells, and no more room is left for the cells to grow as a monolayer. [1]

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