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Large centrifuges are used to simulate high gravity or acceleration environments (for example, high-G training for test pilots). Medium-sized centrifuges are used in washing machines and at some swimming pools to draw water out of fabrics. Gas centrifuges are used for isotope separation, such as to enrich nuclear fuel for fissile isotopes.
Density gradients for purifying cells are also run in these centrifuges. Swinging-bucket rotors tend to be used very widely because of the huge flexibility of sample size through the use of adaptors. [9] These machines have maximum rotor speeds of less than 10 000 rpm and vary from small, bench-top to large, floor-standing centrifuges. [11]
A tricanter centrifuge operates on a similar principle to decanter centrifuges but instead separates three phases, consisting of a suspended solids phase and two immiscible liquids. [5] Sedimentation of the suspended solids occurs as normal where they accumulate on the wall of the bowl and are conveyed out of the centrifuge.
A laboratory centrifuge is a piece of laboratory equipment, driven by a motor, which spins liquid samples at high speed. There are various types of centrifuges, depending on the size and the sample capacity.
[1] Peeler centrifuge operates on the principle of centrifugal force to separate solids from liquids by density difference. And high rotation speed provides high centrifugal force that allows the suspended solid in feed to settle on the inner surface of drum, also washing and washing processes at the same rotational speed and in same centrifuge vessel.
Benchtop fixed-angle centrifuge, or swinging bucket centrifuge Intact (eukaryotic) cells, macroscopic debris Varies depending on sample Gently lysed cells (e.g. dounce homogenizer) 600 x g 10 min Benchtop fixed-angle centrifuge, or swinging bucket centrifuge Nuclei Cytosol, non-nuclei organelles Supernatant of previous row 15,000 x g 20 min
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