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The higher the cell concentration, the higher the turbidity. Spectrophotometers can measure intensity of light very accurately. The cell culture is placed in a transparent cuvette and the absorption is measured relative to medium alone. Optical density (OD) is directly proportional to the biomass in the cell suspension in a given range that is ...
The mean corpuscular hemoglobin concentration (MCHC) is a measure of the concentration of hemoglobin in a given volume of packed red blood cell. It is calculated by dividing the hemoglobin by the hematocrit. Reference ranges for blood tests are 32 to 36 g/dL (320 to 360g/L), [1] or between 4.81 and 5.58 mmol/L. It is thus a mass or molar ...
The number of cells in the chamber can be determined by direct counting using a microscope, and visually distinguishable cells can be differentially counted. The number of cells in the chamber is used to calculate the concentration or density of the cells in the mixture the sample comes from. It is the number of cells in the chamber divided by ...
Mean corpuscular volume (MCV) is the average volume of a red blood cell and is calculated by dividing the hematocrit (Hct) by the concentration of red blood cell count. [citation needed] = [] Normal range: 80–100 fL (femtoliter)
The mean corpuscular hemoglobin, or "mean cell hemoglobin" (MCH), is the average mass of hemoglobin (Hb) per red blood cell (RBC) in a sample of blood. It is reported as part of a standard complete blood count. MCH value is diminished in hypochromic anemias. [1] RBCs are either normochromic or hypochromic. They are never "hyperchromic".
A dilution of the cells to be counted is prepared and mixed with Trypan blue, this is normally the stain of choice because it is taken up by dead cells and actively excluded from live cells. Once the cells have been stained, they are counted using a hemocytometer, then a calculation is carried out to the original concentration of live cells. [1]
The MCV can be conceptualized as the total volume of a group of cells divided by the number of cells. For a real world sized example, imagine you had 10 small jellybeans with a combined volume of 10 μL. The mean volume of a jellybean in this group would be 10 μL / 10 jellybeans = 1 μL / jellybean. A similar calculation works for MCV.
The measurement depends on the number and size of red blood cells. [3] It is normally 40.7–50.3% for males and 36.1–44.3% for females. [3] It is a part of a person's complete blood count results, [4] along with hemoglobin concentration, white blood cell count and platelet count.