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To identify an saRNA that can activate a gene of interest, several saRNAs need to be designed within a 1- to 2-kbp promoter region by following a set of rules [3] [4] and tested in cultured cells. In some reports, saRNAs are designed in such a way to target non-coding transcripts that overlap the promoter sequence of a protein coding gene.
Mechanism of self-amplifying mRNA (saRNA) used for antigen production. The ORF encoding the antigen can also be substituted with a protein for use in protein replacement therapy. This sequence encodes the protein of interest, used as an antigen in the case of vaccines or for protein replacement therapies. The gene of interest replaces the viral ...
Mozzarella sticks are prepared by coating cheese strings in breadcrumbs or batter. They may be baked or fried in oil. Mozzarella sticks are often served with tomato sauce or marinara sauce. However, they may be served with other dipping sauces such as plum sauce, jalapeño jelly, ketchup, barbecue sauce, honey mustard sauce, and ranch dressing ...
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However, even regular liquorice candy can contain up to 2% ammonium chloride, the taste of which is less prominent because of the higher sugar concentration. [3] Some liquorice candy is flavoured with anise oil instead of or in combination with liquorice root extract, because anise has a very similar flavour.
Skewers are used while grilling or roasting meats and fish, and in other culinary applications. In English, brochette is a borrowing of the French word for skewer. In cookery , en brochette means 'on a skewer', and describes the form of a dish or the method of cooking and serving pieces of food, especially grilled meat or seafood, on skewers ...
The French word profiterole, 'small profit, gratification', has been used in cuisine since the 16th century. [ 6 ] In the 17th century, profiteroles were small hollow bread rolls filled with a mixture of sweetbreads, truffles, artichoke bottoms, mushrooms, pieces of partridge, pheasant, or various poultry, accompanied by garnish.
Candy treats that use roller balls to let kids eat sweet liquid have been linked to choking, including at least one death, and should not be used, a federal safety agency warned Thursday. The U.S ...