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The major spliceosome splices introns containing GU at the 5' splice site and AG at the 3' splice site. It is composed of the U1 , U2 , U4 , U5 , and U6 snRNPs and is active in the nucleus. In addition, a number of proteins including U2 small nuclear RNA auxiliary factor 1 (U2AF35), U2AF2 (U2AF65) [ 10 ] and SF1 are required for the assembly of ...
A splice site mutation is a genetic mutation that inserts, deletes or changes a number of nucleotides in the specific site at which splicing takes place during the processing of precursor messenger RNA into mature messenger RNA. Splice site consensus sequences that drive exon recognition are located at the very termini of introns. [1]
The 5' splice site sequence is then subjected to a nucleophilic attack by a downstream sequence called the branch point, resulting in a circular structure called a lariat. The free 5' exon then attacks the 3' splice site, joining the two exons and releasing a structure known as an intron lariat. The intron lariat is subsequently de-branched and ...
Group I catalytic intron. Group I introns are large self-splicing ribozymes. They catalyze their own excision from mRNA, tRNA and rRNA precursors in a wide range of organisms. [1][2][3] The core secondary structure consists of nine paired regions (P1-P9). [4] These fold to essentially two domains – the P4-P6 domain (formed from the stacking ...
Alternative splicing produces three protein isoforms. Protein A includes all of the exons, whereas Proteins B and C result from exon skipping. Alternative splicing, or alternative RNA splicing, or differential splicing, is an alternative splicing process during gene expression that allows a single gene to produce different splice variants.
Method. Trapping is performed with gene trap vectors whose principal element is a gene trapping cassette consisting of a promoterless reporter gene and/or selectable genetic marker, flanked by an upstream 3' splice site (splice acceptor; SA) and a downstream transcriptional termination sequence (polyadenylation sequence; polyA). When inserted ...
This data can be used to annotate where expressed genes are, their relative expression levels, and any alternative splice variants. [ 1 ] RNA-Seq (named as an abbreviation of RNA sequencing ) is a technique that uses next-generation sequencing to reveal the presence and quantity of RNA molecules in a biological sample, providing a snapshot of ...
The outron is an intron-like sequence possessing similar characteristics such as the G+C content [3] and a splice acceptor site that is the signal for trans-splicing. [4] [5] Such a trans-splice site is essentially defined as an acceptor (3') splice site without an upstream donor (5') splice site.