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  2. Somatic embryogenesis - Wikipedia

    en.wikipedia.org/wiki/Somatic_embryogenesis

    Switchgrass somatic embryos. Somatic embryogenesis is an artificial process in which a plant or embryo is derived from a single somatic cell. [1] Somatic embryos are formed from plant cells that are not normally involved in the development of embryos, i.e. ordinary plant tissue. No endosperm or seed coat is formed around a somatic embryo.

  3. Somatic cell nuclear transfer - Wikipedia

    en.wikipedia.org/wiki/Somatic_cell_nuclear_transfer

    What is left is a somatic cell and an enucleated egg cell. These are then fused by inserting the somatic cell into the 'empty' ovum. [7] After being inserted into the egg, the somatic cell nucleus is reprogrammed by its host egg cell. The ovum, now containing the somatic cell's nucleus, is stimulated with a shock and will begin to divide.

  4. Plant tissue culture - Wikipedia

    en.wikipedia.org/wiki/Plant_tissue_culture

    Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation .

  5. Plant embryonic development - Wikipedia

    en.wikipedia.org/wiki/Plant_embryonic_development

    Plant embryonic development, also plant embryogenesis, is a process that occurs after the fertilization of an ovule to produce a fully developed plant embryo.This is a pertinent stage in the plant life cycle that is followed by dormancy and germination. [1]

  6. Cell culture - Wikipedia

    en.wikipedia.org/wiki/Cell_culture

    Tissue culture is an important tool for the study of the biology of cells from multicellular organisms. It provides an in vitro model of the tissue in a well defined environment which can be easily manipulated and analysed. In animal tissue culture, cells may be grown as two-dimensional monolayers (conventional culture) or within fibrous ...

  7. Embryo culture - Wikipedia

    en.wikipedia.org/wiki/Embryo_culture

    Single or sequential medium are equally effective for the culture of human embryos to the blastocyst stage. [7] Artificial embryo culture media basically contain glucose, pyruvate, and energy-providing components, but the addition of amino acids, nucleotides, vitamins, and cholesterol improve the performance of embryonic growth and development.

  8. Stem-cell line - Wikipedia

    en.wikipedia.org/wiki/Stem-cell_line

    Culture conditions such as the cell growth medium and surface on which cells are grown vary widely depending on the specific stem cell line. Different biochemical factors can be added to the medium to control the cell phenotype—for example to keep stem cells in a pluripotent state or to differentiate them to a specific cell type.

  9. Embryo rescue - Wikipedia

    en.wikipedia.org/wiki/Embryo_rescue

    This technique nurtures the immature or weak embryo, thus allowing it the chance to survive. Plant embryos are multicellular structures that have the potential to develop into a new plant. The most widely used embryo rescue procedure is referred to as embryo culture, and involves excising plant embryos and placing them onto media culture. [2]