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2. Stem-cell line - Wikipedia

   en.wikipedia.org/wiki/Stem-cell_line

   A stem cell line is a group of stem cells that is cultured in vitro and can be propagated indefinitely. Stem cell lines are derived from either animal or human tissues and come from one of three sources: embryonic stem cells, adult stem cells, or induced pluripotent stem cells. They are commonly used in research and regenerative medicine.

3. Embryonic stem cell - Wikipedia

   en.wikipedia.org/wiki/Embryonic_stem_cell

   These cells are fed daily and are enzymatically or mechanically separated every four to seven days. For differentiation to occur, the human embryonic stem cell line is removed from the supporting cells to form embryoid bodies, is co-cultured with a serum containing necessary signals, or is grafted in a three-dimensional scaffold to result. [63]

4. James Thomson (cell biologist) - Wikipedia

   en.wikipedia.org/wiki/James_Thomson_(cell_biologist)

   In 1998, Thomson's Lab was the first to report the successful isolation of human embryonic stem cells. On November 6, 1998, Science published this research in an article titled "Embryonic Stem Cell Lines Derived from Human Blastocysts", results which Science later featured in its “Scientific Breakthrough of the Year” article, 1999. [3]

5. Stem cell - Wikipedia

   en.wikipedia.org/wiki/Stem_cell

   Since 1998 however, it has been possible to culture and differentiate human embryonic stem cells (in stem-cell lines). The process of isolating these cells has been controversial, because it typically results in the destruction of the embryo.

6. Somatic cell nuclear transfer - Wikipedia

   en.wikipedia.org/wiki/Somatic_cell_nuclear_transfer

   Taking this into account the research group applied their new technique in an attempt to produce human SCNT stem cells. In May 2013, the Oregon group reported the successful derivation of human embryonic stem cell lines derived through SCNT, using fetal and infant donor cells.

7. Mouse embryonic fibroblast - Wikipedia

   en.wikipedia.org/wiki/Mouse_embryonic_fibroblast

   A photo of human embryonic stem cells (the cell colonies in the center). Spindle cells surrounding the stem cell colony are MEFs. Mouse Embryonic Fibroblasts (MEFs) are a type of fibroblast prepared from mouse embryo. MEFs show a spindle shape when cultured in vitro, a typical feature of fibroblasts. The MEF is a limited cell line.