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  2. Variants of PCR - Wikipedia

    en.wikipedia.org/wiki/Variants_of_PCR

    Similarly, thermal asymmetric interlaced PCR (or TAIL-PCR) is used to isolate unknown sequences flanking a known area of the genome. Within the known sequence, TAIL-PCR uses a nested pair of primers with differing annealing temperatures. A 'degenerate' primer is used to amplify in the other direction from the unknown sequence. [27]

  3. Nested polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Nested_polymerase_chain...

    A diagram illustrating the method of nested PCR. Nested polymerase chain reaction ( nested PCR ) is a modification of polymerase chain reaction intended to reduce non-specific binding in products due to the amplification of unexpected primer binding sites.

  4. Multiplex polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Multiplex_polymerase_chain...

    Multiplex-PCR consists of multiple primer sets within a single PCR mixture to produce amplicons of varying sizes that are specific to different DNA sequences. By targeting multiple sequences at once, additional information may be gained from a single test run that otherwise would require several times the reagents and more time to perform.

  5. Polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Polymerase_chain_reaction

    A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.

  6. Overlap extension polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Overlap_extension...

    Second, the formerly obtained PCR products are combined together into the overlap extension PCR reaction, where the complementary overhangs bind pair-wise allowing the polymerase to extend the DNA strand. Eventually, outer primers targeting the external overhangs are used and the desired DNA product is amplified in the final PCR reaction.

  7. Real-time polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Real-time_polymerase_chain...

    A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR.

  8. This type of meningitis is fast and deadly. Here's what to know.

    www.aol.com/type-meningitis-fast-deadly-heres...

    Here's what bacterial meningitis is, how it's different from viral and other types of meningitis, and what treatment can be expected.

  9. Polymerase chain reaction optimization - Wikipedia

    en.wikipedia.org/wiki/Polymerase_chain_reaction...

    The polymerase chain reaction (PCR) is a commonly used molecular biology tool for amplifying DNA, and various techniques for PCR optimization which have been developed by molecular biologists to improve PCR performance and minimize failure.