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Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase. Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released. Hence ...
English: Gray, blue, red, green, light green, black graph papers with 1 cm–0.5 cm–1 mm grids (page size: A4) in printable PDF format. Date 25 July 2013, 18:04:17
Standard flowgram format (SFF) is a binary file format used to encode results of pyrosequencing from the 454 Life Sciences platform for high-throughput sequencing.SFF files can be viewed, edited and converted with DNA Baser SFF Workbench (graphic tool), or converted to FASTQ format with sff2fastq or seq_crumbs.
Pyrosequencing uses luciferase to generate light for detection of the individual nucleotides added to the nascent DNA, and the combined data are used to generate sequence reads. [81] This technology provides intermediate read length and price per base compared to Sanger sequencing on one end and Solexa and SOLiD on the other. [90]
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Hexagonal paper shows regular hexagons instead of squares. These can be used to map geometric tiled or tesselated designs among other uses. Isometric graph paper or 3D graph paper is a triangular graph paper which uses a series of three guidelines forming a 60° grid of small triangles. The triangles are arranged in groups of six to make hexagons.
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This is a method of "sequencing by synthesis", during which a complementary strand is built based on the sequence of a template strand. An Ion Proton semiconductor sequencer. A microwell containing a template DNA strand to be sequenced is flooded with a single species of deoxyribonucleotide triphosphate (dNTP).