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Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase. Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released. Hence ...
English: Gray, blue, red, green, light green, black graph papers with 1 cm–0.5 cm–1 mm grids (page size: A4) in printable PDF format. Date 25 July 2013, 18:04:17
Standard flowgram format (SFF) is a binary file format used to encode results of pyrosequencing from the 454 Life Sciences platform for high-throughput sequencing.SFF files can be viewed, edited and converted with DNA Baser SFF Workbench (graphic tool), or converted to FASTQ format with sff2fastq or seq_crumbs.
English: Gray, blue, red, green, black graph papers with 1 inch–0.5 inch–1/12 inch grids (page size: US Letter) in printable PDF format. Date 25 July 2013, 18:02:35
Pyrosequencing uses luciferase to generate light for detection of the individual nucleotides added to the nascent DNA, and the combined data are used to generate sequence reads. [81] This technology provides intermediate read length and price per base compared to Sanger sequencing on one end and Solexa and SOLiD on the other.
Pyrosequencing, a new method of DNA sequencing which involves the addition of phosphorylated dNTP's to the DNA polymerase reaction which emmit light upon binding to the DNA template. It makes DNA sequencing significantly faster than with the current chain termination method. We do use them, look at Pyrosequencing/Temp.
Hexagonal paper shows regular hexagons instead of squares. These can be used to map geometric tiled or tesselated designs among other uses. Isometric graph paper or 3D graph paper is a triangular graph paper which uses a series of three guidelines forming a 60° grid of small triangles. The triangles are arranged in groups of six to make hexagons.
A process flow diagram (PFD) is a diagram commonly used in chemical and process engineering to indicate the general flow of plant processes and equipment. The PFD displays the relationship between major equipment of a plant facility and does not show minor details such as piping details and designations.
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