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Amplified fragment length polymorphism (AFLP-PCR or AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990s by KeyGene, [1] AFLP uses restriction enzymes to digest genomic DNA, followed by ligation of adaptors to the sticky ends of the restriction fragments.
DNA profiling is a forensictechnique in criminal investigations, comparing criminal suspects' profiles to DNA evidence so as to assess the likelihood of their involvement in the crime. [1][2]It is also used in paternity testing,[3]to establish immigration eligibility,[4]and in genealogicaland medical research.
DNA footprinting is a method of investigating the sequence specificity of DNA -binding proteins in vitro. This technique can be used to study protein-DNA interactions both outside and within cells. The regulation of transcription has been studied extensively, and yet there is still much that is unknown.
The method can also be applied to non-human species, for example in wildlife population genetics studies. [20] Before his methods were commercialised in 1987, his laboratory was the only centre in the world that carried out DNA fingerprinting, and was consequently very busy, receiving inquiries from all over the globe. [9] [19]
The northern blot, or RNA blot, [1] is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample. [2][3] With northern blotting it is possible to observe cellular control over structure and function by determining the particular gene expression rates during differentiation and ...
In molecular biology, restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences, known as polymorphisms, populations, or species or to pinpoint the locations of genes within a sequence. The term may refer to a polymorphism itself, as detected through the differing locations of ...
Centre for DNA Fingerprinting and Diagnostics (CDFD), Uppal campus. CDFD was conceptualised by then CCMB director Lalji Singh. It evolved into its current form of a modern institution encompassing both basic and applied research in diverse areas of modern biology under its founder director, Seyed E. Hasnain (former vice-chancellor of the University of Hyderabad), who pursued this aim ...
Ribosomal RNA ( rRNA) intergenic spacer analysis ( RISA) is a method of microbial community analysis that provides a means of comparing differing environments or treatment impacts without the bias imposed by culture- dependent approaches. [1] RISA involves PCR amplification of a region of the rRNA gene operon between the small ( 16S) and large ...