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A bromophenol is an organic compound consisting of hydroxyl groups and bromine atoms bonded to a benzene ring. They may be viewed as hydroxyl derivatives of bromobenzene, or as brominated derivatives of phenol. There are five basic types of bromophenols (mono- to pentabromophenol) and 19 different bromophenols in total when positional isomerism ...
The monobromophenols are chemical compounds consisting of phenol substituted with a bromine atom. There are three isomers, 2-bromophenol, 3-bromophenol, and 4-bromophenol. Bromophenols. IUPAC name. 2-Bromophenol. 3-Bromophenol.
Bromophenol is also used as a colour marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis.Since bromophenol blue carries a slight negative charge at moderate pH, it will migrate in the same direction as DNA or protein in a gel; the rate at which it migrates varies according to gel density and buffer composition, but in a typical 1% agarose gel in ...
Isosbestic points are used in medicine in a laboratory technique called oximetry to determine hemoglobin concentration, regardless of its saturation. Oxyhaemoglobin and deoxyhaemoglobin have (not exclusively) isosbestic points at 586 nm and near 808 nm. Isosbestic points are also used in clinical chemistry, as a quality assurance method, to ...
Dissociation constant. In chemistry, biochemistry, and pharmacology, a dissociation constant (KD) is a specific type of equilibrium constant that measures the propensity of a larger object to separate (dissociate) reversibly into smaller components, as when a complex falls apart into its component molecules, or when a salt splits up into its ...
A small percentage of the intermediate loses the chloride to become the product (2,4-dinitrophenol, 3), while the rest return to the reactant (1). Since 2,4-dinitrophenol is in a lower energy state, it will not return to form the reactant, so after some time has passed, the reaction reaches chemical equilibrium that favors the 2,4-dinitrophenol ...
The SI unit of molar absorption coefficient is the square metre per mole (m 2 /mol), but in practice, quantities are usually expressed in terms of M −1 ⋅cm −1 or L⋅mol −1 ⋅cm −1 (the latter two units are both equal to 0.1 m 2 /mol). In older literature, the cm 2 /mol is sometimes used; 1 M −1 ⋅cm −1 equals 1000 cm 2 /mol.
Mass fraction (chemistry) In chemistry, the mass fraction of a substance within a mixture is the ratio (alternatively denoted ) of the mass of that substance to the total mass of the mixture. [1] Expressed as a formula, the mass fraction is: Because the individual masses of the ingredients of a mixture sum to , their mass fractions sum to unity: