Search results
Results from the WOW.Com Content Network
During the period of exponential DNA increase at 37 °C, the rate of strand elongation was 749 nucleotides per second. The mutation rate per base pair per round of replication during phage T4 DNA synthesis is 2.4 × 10 −8. [11] Thus, semiconservative DNA replication is both rapid and accurate.
During DNA replication, the replisome will unwind the parental duplex DNA into a two single-stranded DNA template replication fork in a 5' to 3' direction. The leading strand is the template strand that is being replicated in the same direction as the movement of the replication fork.
Eukaryotes initiate DNA replication at multiple points in the chromosome, so replication forks meet and terminate at many points in the chromosome. Because eukaryotes have linear chromosomes, DNA replication is unable to reach the very end of the chromosomes. Due to this problem, DNA is lost in each replication cycle from the end of the chromosome.
Main page; Contents; Current events; Random article; About Wikipedia; Contact us; Pages for logged out editors learn more
After that, E. coli cells with only 15 N in their DNA were transferred to a 14 N medium and were allowed to divide; the progress of cell division was monitored by microscopic cell counts and by colony assay. DNA was extracted periodically and was compared to pure 14 N DNA and 15 N DNA. After one replication, the DNA was found to have ...
The process of duplicating DNA is called DNA replication, and it takes place by first unwinding the duplex DNA molecule, starting at many locations called DNA replication origins, followed by an unzipping process that unwinds the DNA as it is being copied. However, replication does not start at all the different origins at once.
DNA is a duplex formed by two anti-parallel strands. Following Meselson-Stahl, the process of DNA replication is semi-conservative, whereby during replication the original DNA duplex is separated into two daughter strands (referred to as the leading and lagging strand templates). Each daughter strand becomes part of a new DNA duplex.
After one generation of growth, all the DNA was seen to have a density halfway between that of 15 N DNA and 14 N DNA. In successive generations, the fraction of DNA that was "half-heavy" fell by a factor of ½, as the total amount of DNA increased two-fold. When the half-heavy DNA was made single stranded by heating, it separated into two ...