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In strongly basic solutions, phenolphthalein is converted to its In(OH) 3− form, and its pink color undergoes a rather slow fading reaction [6] and becomes completely colorless when pH is greater than 13. The pK a values of phenolphthalein were found to be 9.05, 9.50 and 12 while those of phenolsulfonphthalein are 1.2 and 7.70. [2]
Normally, the indicator causes the color of the solution to change depending on the pH. Indicators can also show change in other physical properties; for example, olfactory indicators show change in their odor. The pH value of a neutral solution is 7.0 at 25°C (standard laboratory conditions). Solutions with a pH value below 7.0 are considered ...
Colorimetric assays use reagents that undergo a measurable color change in the presence of the analyte. They are widely used in biochemistry to test for the presence of enzymes, specific compounds, antibodies, hormones and many more analytes. For example, para-Nitrophenylphosphate is converted into a yellow product by alkaline phosphatase enzyme.
Its sharp and easily detectable colour changes makes phenolphthalein a valuable tool for determining the endpoint of acid-base titrations, as a precise pH change signifies the completion of the reaction. When a weak acid reacts with a weak base, the equivalence point solution will be basic if the base is stronger and acidic if the acid is stronger.
A complexometric indicator is an ionochromic dye that undergoes a definite color change in presence of specific metal ions. [1] It forms a weak complex with the ions present in the solution, which has a significantly different color from the form existing outside the complex. Complexometric indicators are also known as pM indicators.
A pH indicator is a substance that changes color in response to a chemical change. An acid-base indicator (e.g., phenolphthalein) changes color depending on the pH. Redox indicators are also frequently used. A drop of indicator solution is added to the titration at the start; when the color changes the endpoint has been reached, this is an ...
In a titration of a weak acid with a strong base the pH rises more steeply as the end-point is approached. At the end-point, the slope of the curve of pH with respect to amount of titrant is a maximum. Since the end-point occurs at pH greater than 7, the most suitable indicator to use is one, like phenolphthalein, that changes color at high pH. [2]
The meter reading (in millivolts) is plotted against the volume of titrant. The end point is taken at the distinct inflection of the resulting titration curve corresponding to the basic buffer solution. Color indicating titration: An appropriate pH color indicator e.g. phenolphthalein, is used. Titrant is added to the sample by means of a burette.