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The actual power or magnification of a compound optical microscope is the product of the powers of the eyepiece and the objective lens. For example a 10x eyepiece magnification and a 100x objective lens magnification gives a total magnification of 1,000×.
The ratio of the focal length of the objective and the eyepiece, when mounted in a standard tube length, gives an approximate magnification of the system. Due to their design, compound microscopes have improved resolving power and contrast in comparison to simple microscopes, [ 11 ] and can be used to view the structure, shape and motility of a ...
One of the most important properties of microscope objectives is their magnification. The magnification typically ranges from 4× to 100×. It is combined with the magnification of the eyepiece to determine the overall magnification of the microscope; a 4× objective with a 10× eyepiece produces an image that is 40 times the size of the object.
An ocular micrometer or eyepiece micrometer is a glass disk, engraved with a ruled scale, that fits in an eyepiece of a microscope, [1] [2] which is used to measure the size of microscopic objects through magnification under a microscope. When the eyepiece micrometer is calibrated using a stage micrometer, the length of the divisions on the ...
By convention, for magnifying glasses and optical microscopes, where the size of the object is a linear dimension and the apparent size is an angle, the magnification is the ratio between the apparent (angular) size as seen in the eyepiece and the angular size of the object when placed at the conventional closest distance of distinct vision: 25 ...
The eye relief of an optical instrument (such as a telescope, a microscope, or binoculars) is the distance from the last surface of an eyepiece within which the user's eye can obtain the full viewing angle. If a viewer's eye is outside this distance, a reduced field of view will be obtained.
Some stereo microscopes can deliver a useful magnification up to 100×, comparable to a 10× objective and 10× eyepiece in a normal compound microscope, although the magnification is often much lower. This is around one tenth the useful resolution of a normal compound optical microscope.
Area per high-power field for some microscope types: Olympus BX50, BX40 or BH2 or AO: 0.096 mm 2 [1] AO with 10x eyepiece: 0.12 mm 2 [1] Olympus with 10x eyepiece: 0.16 mm 2 [1] Nikon Eclipse E400 with 10x eyepiece and 40x objective: 0.25mm 2 [2] Leitz Ortholux: 0.27 mm 2 [1] Leitz Diaplan: 0.31 mm 2 [1]
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