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The F-plasmid (first named F by one of its discoverers Esther Lederberg；also called the sex factor in E. coli，the F sex factor, or the fertility factor) [1] [2] [3] allows genes to be transferred from one bacterium carrying the factor to another bacterium lacking the factor by conjugation. The F factor was the first plasmid to be discovered ...
Due to the F factor's inherent tendency to transfer itself during conjugation, the rest of the bacterial genome is dragged along with it. Therefore, unlike a normal F + cell, Hfr strains will attempt to transfer their entire DNA through the mating bridge, in a fashion similar to the normal conjugation. In a typical conjugation, the recipient ...
The F-factor is an episome (a plasmid that can integrate itself into the bacterial chromosome by homologous recombination) with a length of about 100 kb. It carries its own origin of replication, the oriV, and an origin of transfer, or oriT. [5] There can only be one copy of the F-plasmid in a given bacterium, either free or integrated, and ...
Perhaps the most well-studied is the F-pilus of Escherichia coli, encoded by the F sex factor. Escherichia coli undergoing conjugation. Bacteria produce long extracellular appendages called sex pili, which connect two neighbouring cells and serve as a physical conduit for transfer of DNA. Adapted from [4] A sex pilus is typically 6 to 7 nm in ...
Since many R-factors contain F-plasmids, antibiotic resistance can be easily spread among a population of bacteria. [19] Also, R-factors can be taken up by "DNA pumps" in their membranes via transformation , [ 20 ] or less commonly through viral mediated transduction , [ 21 ] or via bacteriophage, although conjugation is the most common means ...
A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. coli. [ 1 ] [ 2 ] [ 3 ] F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division.
The donor cell maintains a functional copy of the plasmid. It later was discovered that the F factor was the first episome and can exist as an independent plasmid making it a very stable vector for cloning. Conjugation aids in the formation of bacterial clone libraries by ensuring all cells contain the desired fosmid. [4]
F-factor may refer to: F-factor (conversion factor), a conversion unit used in diagnostic radiology; Fertility factor (bacteria), a sequence of bacterial DNA;