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[28] [29] ELISA can also be used in toxicology as a rapid presumptive screen for certain classes of drugs. Enzyme-linked immunosorbent assay plate. The ELISA was the first screening test widely used for HIV because of its high sensitivity. In an ELISA, a person's serum is diluted 400 times and applied to a plate to which HIV antigens are attached.
Composite microplates, including filter bottom plates, solid phase extraction (SPE) plates, and even some advanced PCR plate designs, use multiple components and/or materials which are moulded separately and later assembled into a finished product. ELISA plates may now be assembled from twelve separate strips of eight wells, making it easier to ...
ELISA plate showing various cortisol levels Enzymes used in ELISAs include horseradish peroxidase (HRP), alkaline phosphatase (AP) or glucose oxidase . These enzymes allow for detection often because they produce an observable color change in the presence of certain reagents.
Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery , [ 1 ] bioassay validation, quality control and manufacturing processes in the pharmaceutical and ...
Instrument Uses Test tube: Folin-Wu tube: Glass slide mycole and cover slips: in microscopy, serology, etc. as the solid backing on which test samples are : Petri dish: used for preparation of culture media and the culture of organisms they are in
In the nuclease hybridization assay, the oligonucleotide analyte is captured onto the solid support such as a 96-well plate via a fully complementary cutting probe. After enzymatic processing by S1 nuclease, the free cutting probe and the cutting probe hybridized to metabolites, i.e. shortmers of the analyte are degraded, allowing signal to be ...
Automatic analysis of urine test strips using automated urine test strip analysers is a well-established practice in modern-day urinalysis. They can measure calcium , blood, glucose, bilirubin, urobilinogen, ketones, leukocytes, creatinine , microalbumin , pH, ascorbic acid and protein.
Microtitre plates: mostly used for ELISA: Microtome: cuts prepared specimens for analysis under microscope Nichrome wire loop: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc.; sterilized by flaming to red hot before use Petri dish/agar plate: to act as a supporting container to hold the ...
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