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  2. File:ELISA-sandwich.svg - Wikipedia

    en.wikipedia.org/wiki/File:ELISA-sandwich.svg

    Description: A sandwich ELISA. (1) Plate is coated with a capture antibody; (2) sample is added, and any antigen present binds to capture antibody; (3) detecting antibody is added, and binds to antigen; (4) enzyme-linked secondary antibody is added, and binds to detecting antibody; (5) substrate is added, and is converted by enzyme to detectable form.

  3. ELISA - Wikipedia

    en.wikipedia.org/wiki/ELISA

    A sandwich ELISA. (1) Plate is coated with a capture antibody; (2) sample is added, and any antigen present binds to capture antibody; (3) detecting antibody is added, and binds to antigen; (4) enzyme-linked secondary antibody is added, and binds to detecting antibody; (5) substrate is added, and is converted by enzyme into a detectable form.

  4. File:Sandwich Immunoassay, ELISA.jpg - Wikipedia

    en.wikipedia.org/wiki/File:Sandwich_Immunoassay...

    Main page; Contents; Current events; Random article; About Wikipedia; Contact us

  5. Immunoassay - Wikipedia

    en.wikipedia.org/wiki/Immunoassay

    The amount of labelled antibody on the site is then measured. It will be directly proportional to the concentration of the analyte because the labelled antibody will not bind if the analyte is not present in the unknown sample. This type of immunoassay is also known as a sandwich assay as the analyte is "sandwiched" between two antibodies.

  6. Enzyme immunoassay - Wikipedia

    en.wikipedia.org/wiki/Enzyme_immunoassay

    Enzyme-linked immunosorbent assay (ELISA) Enzyme multiplied immunoassay technique (EMIT) Fluorescent enzyme immunoassays (FEIAs) Chemiluminescent immunoassays (CLIAs)

  7. Hook effect - Wikipedia

    en.wikipedia.org/wiki/Hook_effect

    The effect can also occur because of antigen excess, when both the capture and detection antibodies become saturated by the high analyte concentration. In this case, no sandwich can be formed by the capturing antibody, the antigen and the detection antibody.

  8. Fans Are Worried About Giada De Laurentiis: 'Someone ... - AOL

    www.aol.com/fans-worried-giada-laurentiis...

    Maybe she was going for shock value? Fans have flooded Giada De Laurentiis' latest TikTok video to express their concern after the former Food Network star shared a, well, surprising new recipe to ...

  9. Virus quantification - Wikipedia

    en.wikipedia.org/wiki/Virus_quantification

    ELISA Diagram. Enzyme-linked immunosorbent assay is an antibody-based assay that utilizes an antigen-specific antibody chemically linked to an enzyme (or bound to a second antibody linked to an enzyme) to detect the presence of an unknown amount of the antigen (e.g., viral protein) in a sample.

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