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Polyacrylamide Names IUPAC name. poly(2-propenamide) Other names ... The technique is widely accepted today, and remains a common protocol in molecular biology labs.
Polyacrylamide, the original homologue; Poly(N-isopropylacrylamide), i.e. the well-known PNIPAM or PNIPAAm This page was last edited on 16 ...
The most common liquid polyacrylamide is supplied as an emulsion with 10-40% actives and the rest is a non-aqueous carrier fluid, surfactants and latex. This form allows easy handling of viscous polymers at high concentrations.
Acrylamide (or acrylic amide) is an organic compound with the chemical formula CH 2 =CHC(O)NH 2.It is a white odorless solid, soluble in water and several organic solvents. From the chemistry perspective, acrylamide is a vinyl-substituted primary amide (CONH 2).
The synthesis of poly(N-isopropylacrylamide) began with the synthesis of the acrylamide monomer by Sprecht in 1956. [13]In 1957, Shearer patented the first application for what would be later identified as PNIPA for the use as a rodent repellent. [14]
Polyacrylamide gels are usually used for proteins and have very high resolving power for small fragments of DNA (5-500 bp). Agarose gels, on the other hand, have lower resolving power for DNA but have a greater range of separation, and are therefore used for DNA fragments of usually 50–20,000 bp in size, but the resolution of over 6 Mb is ...
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Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility. Electrophoretic mobility is a function of the length, conformation, and ...