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Polarizing microscope operating principle Depiction of internal organs of a midge larva via birefringence and polarized light microscopy. Polarized light microscopy can mean any of a number of optical microscopy techniques involving polarized light.
Fluorescence polarization immunoassay (FPIA) is a class of in vitro biochemical test used for rapid detection of antibody or antigen in sample. FPIA is a competitive homogenous assay , that consists of a simple prepare and read method, without the requirement of separation or washing steps.
Optical rotation, also known as polarization rotation or circular birefringence, is the rotation of the orientation of the plane of polarization about the optical axis of linearly polarized light as it travels through certain materials.
Doubly refracted image as seen through a calcite crystal, seen through a rotating polarizing filter illustrating the opposite polarization states of the two images. A mathematical description of wave propagation in a birefringent medium is presented below. Following is a qualitative explanation of the phenomenon.
A polarized 3D system uses polarization glasses to create the illusion of three-dimensional images by restricting the light that reaches each eye (an example of stereoscopy). To present stereoscopic images and films, two images are projected superimposed onto the same screen or display through different polarizing filters. The viewer wears low ...
P-polarization is commonly referred to as transverse-magnetic (TM), and has also been termed pi-polarized or π-polarized, or tangential plane polarized. S-polarization is also called transverse-electric (TE), as well as sigma-polarized or σ-polarized, or sagittal plane polarized.
This will happen only when the analyzer is rotated by the same angle by which the plane of polarization of light is rotated by the optically active solution. The position of the analyzer is again noted. The difference of the two readings will give the angle of rotation of the plane of polarization.
Fluorescence anisotropy or fluorescence polarization is the phenomenon where the light emitted by a fluorophore has unequal intensities along different axes of polarization. Early pioneers in the field include Aleksander Jablonski , Gregorio Weber , [ 1 ] and Andreas Albrecht. [ 2 ]