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An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
For example, variations in the pH of the medium can affect the activity of certain antibiotics, and the presence of certain nutrients can promote the growth of specific bacterial species. More so, careful selection and preparation of Mueller Hinton agar is important for accurate microbiological assays.
Streak the mixed culture back and forth in the first quadrant (top left) of the agar plate. Do not cut the agar, simply scrape the top. Flame the loop to rid of culture residue. Wait for it to cool for the next quadrant. Streaking again. Proceed to the second quadrant with streaking. Streaks on the medium will overlap.
Thus, the plate can be used either to estimate the concentration of organisms in a liquid culture or a suitable dilution of that culture using a colony counter, or to generate genetically pure cultures from a mixed culture of genetically different organisms. Several methods are available to plate out cells. One technique is known as "streaking".
A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. Microbial cultures are foundational and basic diagnostic methods used as research tools in molecular biology .
Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, particularly the Gram-negative species Legionella pneumophila. [1] It has also been used for the laboratory diagnosis of Acanthamoeba keratitis, [2] Francisella and Nocardia spp. It contains L-cysteine amino acid and ...
MacConkey agar is a selective and differential culture medium for bacteria. It is designed to selectively isolate gram-negative and enteric (normally found in the intestinal tract) bacteria and differentiate them based on lactose fermentation . [ 1 ]
Sabouraud agar used in combination with additional media, such as Inhibitory Mold Agar (IMA), improves identification of fungal clinical isolates. [8] References