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“As the first company to offer the ESP Detection Option on a cell sorter, we are setting a new standard for small particle research,” said Ming Yan, Ph.D., CTO of Cytek Biosciences. “The enhanced capabilities of our Cytek Aurora CS system not only push the boundaries of scientific discovery but also may open up new markets for Cytek.
Micrograph of paper autofluorescing under ultraviolet illumination. The individual fibres in this sample are around 10 μm in diameter.. Autofluorescence is the natural fluorescence of biological structures such as mitochondria and lysosomes, in contrast to fluorescence originating from artificially added fluorescent markers (fluorophores).
These fluorescence can result in artifacts or background noise in the image. Noise from other fluorescence compounds can be effectively eliminated by using filters to capture the desired fluorescence wavelength, or by making sure the autofluorescence compounds are not present in the sample.
Brightfield DIC image showing guard cells and pavement cells (above). Same region showing Chlorophyll A autofluorescence with 440 nm laser excitation and far red emission (below). Microscopic images of a moss leaf from Plagiomnium undulatum. Bright-field microscopy at the top and fluorescence microscopy at the bottom. The red fluorescence is ...
Aurora A activity is positively-regulated by the spindle protein TPX2, [4] [5] and has recently been shown to be a target for thiol-containing molecules, such as Coenzyme A. [6] Aurora B (a.k.a. Aurora 1) functions in the attachment of the mitotic spindle to the centromere. Aurora C works in germ-line cells and little is known about its function.
In cytometry, compensation is a mathematical correction of a signal overlap between the channels of the emission spectra of different fluorochromes. [1] [2]The photons emitted by fluorochromes have different energies and wavelengths and as flow cytometers use photomultiplier tubes (PMT) in order to convert the photons into electrons, the detector can register the signal from more than one ...
Extraction Profile for Different Types of Extraction. The extraction curve of % recovery against time can be used to elucidate the type of extraction occurring. Figure 4(a) shows a typical diffusion controlled curve. The extraction is initially rapid, until the concentration at the surface drops to zero, and the rate then becomes much slower.
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