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Bromothymol blue is synthesized by addition of elemental bromine to thymol blue in a solution in glacial acetic acid. [ 6 ] To prepare a solution for use as pH indicator, dissolve 0.10 g in 8.0 cm 3 N/50 (a.k.a. 0.02 Normal ) NaOH and dilute with water to 250 cm 3 .
Solution: The main components of a universal indicator, in the form of a solution, are thymol blue, methyl red, bromothymol blue, and phenolphthalein. This mixture is important because each component loses or gains protons depending upon the acidity or alkalinity of the solution being tested. It is beneficial to use this type of universal ...
It was developed in 1953 by Hugh and Leifson to be utilized in microbiology to determine the way a microorganism metabolizes a carbohydrate such as glucose (dextrose). [1] OF-glucose deeps contain glucose as a carbohydrate, peptones , bromothymol blue indicator for Hugh-Leifson's OF medium or phenol red for King's OF medium, and 0.5% agar .
Thymol blue (thymolsulfonephthalein) is a brownish-green or reddish-brown crystalline powder that is used as a pH indicator. It is insoluble in water but soluble in alcohol and dilute alkali solutions.
Phenolphthalein's common use is as an indicator in acid-base titrations. It also serves as a component of universal indicator, together with methyl red, bromothymol blue, and thymol blue. [3] Phenolphthalein adopts different forms in aqueous solution depending on the pH of the solution.
The Food and Drug Administration (FDA) has revoked the use of Red Dye No. 3 (also known as erythrosine, Red Dye 3, FD&C Red No. 3 and Red No. 3) in food and ingested drugs as of January 15 ...
H + + Bromothymol blue (Blue) → Bromothymol blue-H + (Yellow) Elevated protein concentrations produce slightly elevated specific density results as a consequence of the indicator's protein error; in addition, samples with a pH above 6.5 give lower readings as a result of the indicator's bias.
Bromophenol is also used as a colour marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis.Since bromophenol blue carries a slight negative charge at moderate pH, it will migrate in the same direction as DNA or protein in a gel; the rate at which it migrates varies according to gel density and buffer composition, but in a typical 1% agarose gel in ...
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