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Like GFP and other fluorescent proteins, RFP is a barrel-shaped protein made primarily out of β-sheet motifs; this type of protein fold is commonly known as a β-barrel. The mass of RFP is approximately 25.9 kDa. Its excitation maximum is 558 nm, and its emission maximum is 583 nm. [3]
Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. [1] Its excitation peak is 513 nm and its emission peak is 527 nm. [ 2 ]
Distribution of fluorescent proteins in animals. The hippocampus of a mouse imaged via fluorescence microscopy. Biofluorescent emission spectra from amphibians Example uses of fluorescent proteins for imaging in the life sciences. Fluorescence is used in the life sciences generally as a non-destructive way of tracking or analysing biological ...
The fluorescence of a folded protein is a mixture of the fluorescence from individual aromatic residues. Most of the intrinsic fluorescence emissions of a folded protein are due to excitation of tryptophan residues, with some emissions due to tyrosine and phenylalanine; but disulfide bonds also have appreciable absorption in this wavelength range.
smURFP is a homodimer with absorption and emission maximum of 642 nm and 670 nm, respectively. A tandem dimer smURFP (TDsmURFP) was created and has similar properties to smURFP. smURFP is extremely stable with a protein degradation half-life of 17 hour and 33 hour without and with chromophore (), respectively.
mCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). As an RFP, mCherry was derived from DsRed of Discosoma sea anemones, unlike green fluorescent proteins (GFPs) which are often derived from Aequorea victoria jellyfish. [1]
The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. [ 2 ] [ 3 ] The label GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria and is sometimes called avGFP .
When it is excited at 480 nm, green fluorescence is emitted with a peak of 518 nm. When Kaede is irradiated with UV or violet light, the major absorption peak shifts to 572 nm. When excited at 540 nm, Kaede showed an emission maximum at 582 nm with a shoulder at 627 nm and the 518-nm peak. Red fluorescence is emitted after this photoconversion.